An affinity matrix for small-scale isolation and purification of (MBP) fusion proteins.
Monoclonal anti-MBP is covalently coupled to 1 µm nonporous superparamagnetic particles through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the immunomagnetic isolation of MBP-fusion proteins from cell culture extracts
Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins that specifically interact with the immobilized MBP fusion protein
Binding capacity of 5 μg MBP5*-paramyosin ΔSal fusion protein/mg Anti-MBP Magnetic Beads
Quick, small-scale purification of MBP-fusion proteins enables higher throughput and efficiency
An affinity matrix for small-scale isolation and purification of (MBP) fusion proteins.
Monoclonal anti-MBP is covalently coupled to 1 µm nonporous superparamagnetic particles through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the immunomagnetic isolation of MBP-fusion proteins from cell culture extracts
Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins that specifically interact with the immobilized MBP fusion protein
Binding capacity of 5 μg MBP5*-paramyosin ΔSal fusion protein/mg Anti-MBP Magnetic Beads
Quick, small-scale purification of MBP-fusion proteins enables higher throughput and efficiency