E8036S, Chitin Magnetic Beads - 5 ml

962,12 RON

An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD).

SKU

NEB_E8036S

An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD).

Chitin beads have been prepared having a magnetite core, thereby permitting the magnetic isolation of CBD-fusion proteins from cell culture supernatants
The matrix can be regenerated without loss of binding capacity
Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that interact with the immobilized fusion protein
Binding capacity of 2 mg chitin binding domain protein/ml bed volume
Quick, small-scale purification of CBD-fusion proteins enables higher throughput and efficiency
Removal of CBD-tag during elution typically yields highly pure, native protein

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Description	An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD). Chitin beads have been prepared having a magnetite core, thereby permitting the magnetic isolation of CBD-fusion proteins from cell culture supernatants The matrix can be regenerated without loss of binding capacity Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that interact with the immobilized fusion protein Binding capacity of 2 mg chitin binding domain protein/ml bed volume Quick, small-scale purification of CBD-fusion proteins enables higher throughput and efficiency Removal of CBD-tag during elution typically yields highly pure, native protein