An affinity matrix for the small-scale isolation and purification of maltose binding protein (MBP)-fusion proteins.
Amylose is covalently coupled to a superparamagnetic particle through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the isolation of MBP-fusion proteins from cell culture supernatants
Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that may interact with the immobilized fusion protein
Binding capacity of 10 µg MBP5*-paromyosin ΔSal fusion protein/mg amylose magnetic beads
Quick, small-scale purification of MBP-fusion proteins affords higher throughput and efficiency
An affinity matrix for the small-scale isolation and purification of maltose binding protein (MBP)-fusion proteins.
Amylose is covalently coupled to a superparamagnetic particle through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the isolation of MBP-fusion proteins from cell culture supernatants
Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that may interact with the immobilized fusion protein
Binding capacity of 10 µg MBP5*-paromyosin ΔSal fusion protein/mg amylose magnetic beads
Quick, small-scale purification of MBP-fusion proteins affords higher throughput and efficiency