C2992H, NEB 5-alpha F´ Iq Competent E. coli (High Efficiency) - 20 transformation reactions

1.492,26 RON

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

SKU

NEB_C2992H

Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.

Highlights

DH5α™ derivative
Free of animal products
Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
Tight control of expression by lacl^q allows potentially toxic genes to be cloned
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA2)
Suitable for blue/white screening by α-complementation of the β-galactosidase gene
F' allows cells to be infected with bacteriophage M13 for ssDNA production
Reduced recombination of cloned DNA (recA1)
K12 Strain

Transformation Efficiency

1-3 x 10⁹ cfu/µg pUC19 DNA

Genotype

F´ proA⁺B⁺ lacI^q ∆(lacZ)M15 zzf::Tn10 (Tet^R) / fhuA2∆(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

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Price	1.254,00 RON (preturile sunt fara TVA)
Description	Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications. Highlights DH5α™ derivative Free of animal products Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR) Tight control of expression by lacl^q allows potentially toxic genes to be cloned Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations Resistance to phage T1 (fhuA2) Suitable for blue/white screening by α-complementation of the β-galactosidase gene F' allows cells to be infected with bacteriophage M13 for ssDNA production Reduced recombination of cloned DNA (recA1) K12 Strain Transformation Efficiency 1-3 x 10⁹ cfu/µg pUC19 DNA Genotype F´ proA⁺B⁺ lacI^q ∆(lacZ)M15 zzf::Tn10 (Tet^R) / fhuA2∆(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17