C2988J, NEB 5-alpha Competent E. coli (Subcloning Efficiency) - 48 transformation reactions

Product Information
Chemically competent E. coli cells suitable for subcloning efficiency transformations, such as plasmid transformation or routine subcloning (e.g,. inserting a 1 kb fragment into a 2 kb vector). The efficiency of these cells is not sufficiently high for most other cloning applications. For that, we recommend NEB® 5-alpha Competent E. coli (High Efficiency) (NEB #C2987) which has 1,000-fold higher transformation efficiency.
Highlights
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Efficient transformation of unmethylated DNA derived from PCR, cDNA and many other sources (hsdR)
Reduced recombination of cloned DNA (recA1)
Resistance to phage T1 (fhuA2)
Suitable for blue/white screening by α-complementation of the β-galactosidase gene
K12 Strain
DH5α™ derivative
Free of animal products
Transformation Efficiency
> 1 x 106 cfu/µg pUC19 DNA
Genotype
fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17