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C2925I, dam-/dcm- Competent E.coli - 24 transformation reactions (6 tubes)
1.439,90 RON
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation.
SKU
NEB_C2925I
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
Highlights
- Grow plasmids free of Dam and Dcm methylation
- Free of animal products
- Phage T1 resistant (fhuA31)
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- K12 Strain
Transformation Efficiency
1-3 x 106 cfu/μg pUC19 DNA
Genotype
ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TetS endA1 rspL136 (StrR) dam13::Tn9 (CamR) xylA-5 mtl-1 thi-1 mcrB1 hsdR2
| Price | 1.210,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation. Highlights
Transformation Efficiency 1-3 x 106 cfu/μg pUC19 DNA Genotype ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TetS endA1 rspL136 (StrR) dam13::Tn9 (CamR) xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |
