C2566I,  T7 Express Competent E. coli (High Efficiency) - 24 transformation reactions (6 tubes)

C2566I, T7 Express Competent E. coli (High Efficiency) - 24 transformation reactions (6 tubes)

C2925I,  dam-/dcm- Competent E.coli - 24 transformation reactions (6 tubes)

C2925I, dam-/dcm- Competent E.coli - 24 transformation reactions (6 tubes)

C2925H, dam-/dcm- Competent E. coli - 20 transformation reactions

1.871,87 RON

Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation.

SKU
NEB_C2925H

Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.

Highlights

  • Grow plasmids free of Dam and Dcm methylation
  • Free of animal products
  • Phage T1 resistant (fhuA31)
  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
  • K12 Strain

Transformation Efficiency

1-3 x 106 cfu/μg pUC19 DNA

Genotype

ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TetS endA1 rspL136 (StrR) dam13::Tn9 (CamR) xylA-5 mtl-1 thi-1 mcrB1 hsdR2

Mai multe informatii
Price 1.573,00 RON (preturile sunt fara TVA)
Description

Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.

Highlights

  • Grow plasmids free of Dam and Dcm methylation
  • Free of animal products
  • Phage T1 resistant (fhuA31)
  • Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
  • K12 Strain

Transformation Efficiency

1-3 x 106 cfu/μg pUC19 DNA

Genotype

ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TetS endA1 rspL136 (StrR) dam13::Tn9 (CamR) xylA-5 mtl-1 thi-1 mcrB1 hsdR2