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08-57-00250, SOLIScript® 1-step Probe Kit (250 rxn)
2.110,76 RON
Simple and highly specific cDNA synthesis and qPCR in a single tube
Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip® RNase inhibitor, and nuclease-free water.
- cDNA synthesis up to 60°C for superior specificity
- suitable for singleplex and duplex assays
- reaction set-up and shipment without ice
- high specificity and sensitivity
- one kit for all cyclers
- RNase inhibitor included in kit
SKU
SBD_08-61-00250-2
SOLIScript® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays.
It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube
Applications
- Detection and quantification of RNA targets with qPCR
- DNA/LNA hydrolysis probe based assay
- Suitable for singleplex or duplex reactions
Properties
Sample type: RNA
Product format: Reagent kit
No of tubes: three tubes
Detection type: Probe-based
Reference dye: reference dye based on ROX
Compatible real-time instruments: The Kit is compatible with most qPCR cyclers that do or do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal.
Reagents
5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), internal reference based on ROX dye
40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip® RNase Inhibitor
Water, nuclease free
| Price | 1.773,75 RON (preturile sunt fara TVA) |
|---|---|
| Description |
SOLIScript® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube Applications
Properties Sample type: RNA Product format: Reagent kit No of tubes: three tubes Detection type: Probe-based Reference dye: reference dye based on ROX Compatible real-time instruments: The Kit is compatible with most qPCR cyclers that do or do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Reagents 5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), internal reference based on ROX dye 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip® RNase Inhibitor Water, nuclease free
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