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06-33-00050, SOLIScript® KIT (50 rxn)
530,15 RON
Reverse transcriptase with high thermostability and superior specificity
Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature.
- superior performance with gene-specific primers
- reaction temperatures from 37°C to 60°C
- reaction set-up and shipment at room temperature
- NB! not compatible with random primers
SKU
SBD_06-33-00050
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.
SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatures (up to 60°C).
SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains an RNase H domain with reduced activity.
Applications
- First-strand cDNA synthesis
- RT-PCR
- RT-qPCR
Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme with buffer
Enzyme: SOLIScript® Reverse Transcriptase
Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
SOLIScript® Reverse Transcriptase
10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT
| Price | 445,50 RON (preturile sunt fara TVA) |
|---|---|
| Description |
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase. SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatures (up to 60°C). SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains an RNase H domain with reduced activity. Applications
Properties Sample type: RNA Final product: cDNA (first strand) Product format: Enzyme with buffer Enzyme: SOLIScript® Reverse Transcriptase Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers. Reagents SOLIScript® Reverse Transcriptase 10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT
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