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06-13-00050, FIREScript® RT cDNA synthesis KIT (50 rxn)
566,14 RON
Fast and robust reverse transcriptase for wide range of applications
Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures
- high specificity and yield
- wide reaction temperature from 37°C to 60°C
- fast 15 min reaction time
- a simple 2-vial kit with enzyme and reaction buffer only
- reaction set-up and shipment without ice
SKU
SBD_06-13-00050
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.
This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C.
FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
Applications
- First-strand cDNA synthesis
- RT-PCR
- RT-qPCR
Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme and reaction buffer (separate tubes)
Enzyme: FIREScript® Reverse Transcriptase
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene.
Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
FIREScript® Reverse Transcriptase (200 U/μl)
10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)
| Price | 475,75 RON (preturile sunt fara TVA) |
|---|---|
| Description |
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR). Applications
Properties Sample type: RNA Final product: cDNA (first strand) Product format: Enzyme and reaction buffer (separate tubes) Enzyme: FIREScript® Reverse Transcriptase Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene. Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl. Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers. Reagents FIREScript® Reverse Transcriptase (200 U/μl) 10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)
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