06-15-00200, FIREScript® RT cDNA synthesis KIT (200 rxn)

06-15-00200, FIREScript® RT cDNA synthesis KIT (200 rxn)

06-13-00050, FIREScript® RT cDNA synthesis KIT (50 rxn)

06-13-00050, FIREScript® RT cDNA synthesis KIT (50 rxn)

06-13-00200, FIREScript® RT cDNA synthesis KIT (200 rxn)

2.146,76 RON

Fast and robust reverse transcriptase for wide range of applications

Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures

  • high specificity and yield
  • wide reaction temperature from 37°C to 60°C
  • fast 15 min reaction time
  • a simple 2-vial kit with enzyme and reaction buffer only
  • reaction set-up and shipment without ice
SKU
SBD_06-13-00200

FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.

This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C.

FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

Applications

  • First-strand cDNA synthesis
  • RT-PCR
  • RT-qPCR

Properties

Sample type: RNA

Final product: cDNA (first strand)

Product format: Enzyme and reaction buffer (separate tubes)

Enzyme: FIREScript® Reverse Transcriptase

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene.

Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents

FIREScript® Reverse Transcriptase (200 U/μl)

10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)

 

Mai multe informatii
Price 1.804,00 RON (preturile sunt fara TVA)
Description

FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.

This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C.

FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

Applications

  • First-strand cDNA synthesis
  • RT-PCR
  • RT-qPCR

Properties

Sample type: RNA

Final product: cDNA (first strand)

Product format: Enzyme and reaction buffer (separate tubes)

Enzyme: FIREScript® Reverse Transcriptase

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene.

Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents

FIREScript® Reverse Transcriptase (200 U/μl)

10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)