The Monarch Mag Viral DNA/RNA Extraction Kit provides a rapid and reliable magnetic bead-based process for extracting viral nucleic acids from saliva and respiratory swab samples. The kit combines the efficiency of silica-based nucleic acid purification with the ease of use of magnetic beads. Manual and automated workflows allow samples to be processed in microfuge tubes or 96-well plates. Kit sizes align to 96-well formats (100 preps, 600 preps, and 1800 preps), and the protocol is compatible with high throughput automation on a variety of platforms, including the KingFisher Flex magnetic particle processor and Agilent Bravo and MGISP liquid handler platforms.
Properties |
|
Purification format |
Magnetic bead |
Processing format |
Manual or automated |
Sample purification (representative examples) |
Viral DNA and RNA* from respiratory viruses (enveloped and non-enveloped, dsDNA and ssRNA) |
Sample sources |
Saliva, respiratory swab in viral transport media (VTM)** |
Sample input volume |
Up to 200 μl** |
Carrier supplier |
Poly A carrier RNA*** |
Binding capacity |
Up to 3 μg |
Elution volume |
33–100 μl |
Tested automation platforms |
KingFisher Flex; Agilent Bravo and MGISP liquid handlers |
Compatible downstream applications |
qPCR, RT-qPCR, ddPCR, library prep for NGS |
* Viral DNA and RNA are purified in parallel. Preparation of DNA-free RNA or RNA-free DNA requires further treatment with the appropriate nuclease (not supplied).
** The sample input volume may be scalable to accommodate larger sample volumes. Further workflow optimization may be required.
*** Use of carrier RNA is recommended for recovery of low amounts of viral nucleic acid. Carrier RNA should be omitted if the downstream application utilizes poly(A) RNA enrichment; however, viral nucleic acid recovery may be reduced.
Price | 1.094,50 RON (preturile sunt fara TVA) | ||||||||||||||||||||||
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Description |
The Monarch Mag Viral DNA/RNA Extraction Kit provides a rapid and reliable magnetic bead-based process for extracting viral nucleic acids from saliva and respiratory swab samples. The kit combines the efficiency of silica-based nucleic acid purification with the ease of use of magnetic beads. Manual and automated workflows allow samples to be processed in microfuge tubes or 96-well plates. Kit sizes align to 96-well formats (100 preps, 600 preps, and 1800 preps), and the protocol is compatible with high throughput automation on a variety of platforms, including the KingFisher Flex magnetic particle processor and Agilent Bravo and MGISP liquid handler platforms.
* Viral DNA and RNA are purified in parallel. Preparation of DNA-free RNA or RNA-free DNA requires further treatment with the appropriate nuclease (not supplied).
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