T3031,   Mix & Go! Competent Cells-XJa(DE3) Autolysis™ (10 x 100 ul)

T3031, Mix & Go! Competent Cells-XJa(DE3) Autolysis™ (10 x 100 ul)

T3051,   Mix & Go! Competent Cells-XJb(DE3) Autolysis™ (10 x 100 ul)

T3051, Mix & Go! Competent Cells-XJb(DE3) Autolysis™ (10 x 100 ul)

T3041, Mix & Go! Competent Cells-XJb Autolysis™ (10 x 100 ul)

1.844,50 RON
DESCRIPTION

While there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of E. coli cells. The E. coli XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage λ R gene, encoding the λ lysozyme, also known as λ endolysin, is induced during growth. Cells are harvested intact while the peptidoglycan layer of the cell walls has been protected from digestion by the cytoplasmic membrane. The membrane is, however, amenable to disruption by a brief physico-chemical stress such as a freeze-thaw cycle after harvesting the cells. The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be applied to any number of samples without increasing processing time and labor (unlike sonication or French-press), is reliable and repeatable (unlike lysozyme treatment), and finally, is fully compatible with a wide range of buffers. Additionally, it does not require use of any potentially interfering components such as detergents, commonly found in various lytic buffers. They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter.

SKU
ZR_T3041
HIGHLIGHTS

  • Fast: 80 - 90% of E.coli are lysed in only 10 minutes after harvesting.
  • High Transformation Efficiencies: Achieve 108 - 109 transformants per µg of plasmid DNA.
  • Versatile: Fully compatible with a wide range of buffers for protein purification and other physical methods of lysis.
DESCRIPTION

While there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of E. coli cells. The E. coli XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage λ R gene, encoding the λ lysozyme, also known as λ endolysin, is induced during growth. Cells are harvested intact while the peptidoglycan layer of the cell walls has been protected from digestion by the cytoplasmic membrane. The membrane is, however, amenable to disruption by a brief physico-chemical stress such as a freeze-thaw cycle after harvesting the cells. The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be applied to any number of samples without increasing processing time and labor (unlike sonication or French-press), is reliable and repeatable (unlike lysozyme treatment), and finally, is fully compatible with a wide range of buffers. Additionally, it does not require use of any potentially interfering components such as detergents, commonly found in various lytic buffers. They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter.

TECHNICAL SPECIFICATIONS

Autolysis XJb lysis efficiency is 10-20 % lower than XJa. For optimal lysis, more care needs to be taken when selecting the lysis buffer. However, even very low concentrations of detergent may improve lysis significantly.
Cell Growth A very robust strain, reaching higher OD’s than E. coli K-strains.
DNA Extraction XJb is not optimal for DNA extraction.
DNA Stability This strain is RecA positive.
Genotype F- ompT hsdSB(rB - mB -) gal dcm ΔaraB::ΛR, cat (CmR)
Processing Time 10 minutes
Product Storage -70°C to -80°C
Protein Expression XJb is ideal for recombinant protein expression. It lacks Lon and OmpT proteases, leading to higher protein yields.
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA
Supplemental Info
Mai multe informatii
Price 1.550,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS

  • Fast: 80 - 90% of E.coli are lysed in only 10 minutes after harvesting.
  • High Transformation Efficiencies: Achieve 108 - 109 transformants per µg of plasmid DNA.
  • Versatile: Fully compatible with a wide range of buffers for protein purification and other physical methods of lysis.
DESCRIPTION

While there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of E. coli cells. The E. coli XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage λ R gene, encoding the λ lysozyme, also known as λ endolysin, is induced during growth. Cells are harvested intact while the peptidoglycan layer of the cell walls has been protected from digestion by the cytoplasmic membrane. The membrane is, however, amenable to disruption by a brief physico-chemical stress such as a freeze-thaw cycle after harvesting the cells. The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be applied to any number of samples without increasing processing time and labor (unlike sonication or French-press), is reliable and repeatable (unlike lysozyme treatment), and finally, is fully compatible with a wide range of buffers. Additionally, it does not require use of any potentially interfering components such as detergents, commonly found in various lytic buffers. They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter.

TECHNICAL SPECIFICATIONS

Autolysis XJb lysis efficiency is 10-20 % lower than XJa. For optimal lysis, more care needs to be taken when selecting the lysis buffer. However, even very low concentrations of detergent may improve lysis significantly.
Cell Growth A very robust strain, reaching higher OD’s than E. coli K-strains.
DNA Extraction XJb is not optimal for DNA extraction.
DNA Stability This strain is RecA positive.
Genotype F- ompT hsdSB(rB - mB -) gal dcm ΔaraB::ΛR, cat (CmR)
Processing Time 10 minutes
Product Storage -70°C to -80°C
Protein Expression XJb is ideal for recombinant protein expression. It lacks Lon and OmpT proteases, leading to higher protein yields.
Transformation Efficiency 108 - 109 transformants per µg of plasmid DNA
Supplemental Info
Manufacturer Zymo Research