S1427L, NEBExpress Ni Spin Columns - 25 columns

S1427L, NEBExpress Ni Spin Columns - 25 columns

E7860S, NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads - 6 rxns

E7860S, NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads - 6 rxns

S1428S, NEBExpress Ni Resin - 25 ml

2.627,52 RON

NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications.

SKU
NEB_S1428S

NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface.  It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.

Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm.

Storage Buffer

20% Ethanol

Support Matrix

Spherical, agarose based microparticles ranging in size from 10-100 μm.

Binding Capacity

1 ml of NEBExpress™ Ni Resin will bind ≥ 10 mg of His-tagged fusion protein.

Advantages and Features

Features

    • Isolation and purification of His-tagged fusion proteins under native or denaturing conditions as IMAC tolerates a wide range of conditions, including the presence of protein denaturants, reducing agents and detergents.
    • High specific binding of His-tagged proteins from various expression systems yielding milligram quantities of target protein with purities of >95%.
    • Can be used with common cell lysis reagents and a variety of buffer additives

 

 

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Price 2.208,00 RON (preturile sunt fara TVA)
Description

NEBExpress® Ni Resin is an affinity matrix for the isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. It is intended for use in gravity or pressure flow columns, and batch purifications. NEBExpress Ni Resin is comprised of a highly uniform and stable chemical-tolerant resin, pre-charged with nickel ions on the matrix surface.  It is resistant to a wide range of chemicals, including NaOH, EDTA, DTT and β-Mercaptoethanol.

Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm.

Storage Buffer

20% Ethanol

Support Matrix

Spherical, agarose based microparticles ranging in size from 10-100 μm.

Binding Capacity

1 ml of NEBExpress™ Ni Resin will bind ≥ 10 mg of His-tagged fusion protein.

Advantages and Features

Features

    • Isolation and purification of His-tagged fusion proteins under native or denaturing conditions as IMAC tolerates a wide range of conditions, including the presence of protein denaturants, reducing agents and detergents.
    • High specific binding of His-tagged proteins from various expression systems yielding milligram quantities of target protein with purities of >95%.
    • Can be used with common cell lysis reagents and a variety of buffer additives

 

 


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