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S1427S, NEBExpress Ni Spin Columns - 10 columns
871,08 RON
Spin columns containing an affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. Immobilized Metal Affinity Chromatography (IMAC) purifications employing NEBExpress® Ni Spin columns can be performed under native or denaturing conditions, yielding highly pure target in a single protein purification step
SKU
NEB_S1427S
Spin columns containing an affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. Immobilized Metal Affinity Chromatography (IMAC) purifications employing NEBExpress® Ni Spin columns can be performed under native or denaturing conditions, yielding highly pure target in a single protein purification step. This enables screening of expression conditions and streamlines the functional and structural characterization of the target protein.
Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm.
Storage Buffer
20% Ethanol
Support Matrix
Spherical, agarose based microparticles ranging in size from 10-100 μm.
Binding Capacity
Varies with target, ≥ 1 mg His-tagged fusion protein per column.
- Advantages and Features
Features
- Isolation and purification of His-tagged fusion proteins under native or denaturing conditions as IMAC tolerates a wide range of conditions, including the presence of protein denaturants, reducing agents and detergents.
- High specific binding of His-tagged proteins from various expression systems yielding milligram quantities of target protein with purities of >95%.
- Can be used with common cell lysis reagents and a variety of buffer additives
Mai multe informatii
| Price | 732,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Spin columns containing an affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins. Immobilized Metal Affinity Chromatography (IMAC) purifications employing NEBExpress® Ni Spin columns can be performed under native or denaturing conditions, yielding highly pure target in a single protein purification step. This enables screening of expression conditions and streamlines the functional and structural characterization of the target protein. Support Matrix: Spherical, agarose based microparticles ranging in size from 10-100 μm. Storage Buffer 20% Ethanol Support Matrix Spherical, agarose based microparticles ranging in size from 10-100 μm. Binding Capacity Varies with target, ≥ 1 mg His-tagged fusion protein per column.
Features
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