RT-RTCK-05,  Reverse Transcriptase Core Kit 500,  500 RXN

RT-RTCK-05, Reverse Transcriptase Core Kit 500, 500 RXN

RT-UTPUNG-020, dUTP/UNG Additive for dTTP kit conversion - 750 rxns

RT-UTPUNG-020, dUTP/UNG Additive for dTTP kit conversion - 750 rxns

RT-UTPUNG-005, dUTP/UNG Additive for dttp kit conv., 40 µl - 150 rxns

213,39 RON
RT-UTPUNG-005, dUTP/UNG Additive for dttp kit conv., 40 µl - 150 rxns
SKU
EUR_RT-UTPUNG-005

E. coli Uracil-N-glycosylase (UNG) hydrolyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases).

By using the dUTP/UNG blend, easily convert any dTTP MasterMix into a carryover prevention compatible kit in a single pipetting step.

Procedure
Convert a dTTP MasterMix kit before first use:
- Thaw the MasterMix and the vial containing dUTP/UNG at RT
- Add the dUTP/UNG blend to the MasterMix vial as follow:

- 40 µL into 1.5 mL of 2X MasterMix
- 40 µL into 0.6 mL of 5X MasterMix
- 67 µL per 1 mL tube of 5X MasterMix
- 200 µL per 7.5 mL bottle of 2X MasterMix

- Gently homogenize the MasterMix by swirling the vial
- Follow MasterMix storage recommendation for the converted kit

Convert a qPCR reaction into a carryover ready assay (for Core kit):
- Thaw all required reagents
- Add 0.25 µL of dUTP/UNG per 20 µL reaction (final volume - refer to the TDS of the corresponding Core kit)
- Gently homogenize the Mix by pipetting or inverting the tube a few times. Spin down.
- Start the reaction

Core kits always contain dUTP/UNG blend. No need to buy extra kit converter.

 

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Price 179,32 RON (preturile sunt fara TVA)
Description

E. coli Uracil-N-glycosylase (UNG) hydrolyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyses single or double-stranded DNA, but not from oligomers (6 or less bases).

By using the dUTP/UNG blend, easily convert any dTTP MasterMix into a carryover prevention compatible kit in a single pipetting step.

Procedure
Convert a dTTP MasterMix kit before first use:
- Thaw the MasterMix and the vial containing dUTP/UNG at RT
- Add the dUTP/UNG blend to the MasterMix vial as follow:

- 40 µL into 1.5 mL of 2X MasterMix
- 40 µL into 0.6 mL of 5X MasterMix
- 67 µL per 1 mL tube of 5X MasterMix
- 200 µL per 7.5 mL bottle of 2X MasterMix

- Gently homogenize the MasterMix by swirling the vial
- Follow MasterMix storage recommendation for the converted kit

Convert a qPCR reaction into a carryover ready assay (for Core kit):
- Thaw all required reagents
- Add 0.25 µL of dUTP/UNG per 20 µL reaction (final volume - refer to the TDS of the corresponding Core kit)
- Gently homogenize the Mix by pipetting or inverting the tube a few times. Spin down.
- Start the reaction

Core kits always contain dUTP/UNG blend. No need to buy extra kit converter.