R3733L, BsaI-HFv2 - 5.000 units

BsaI-HFv2 is a Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNA cutting by BsaI.

High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (rCutSmart™ Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

• Engineered for improved performance
• Optimized for Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer often used in this application
• Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
• 100% activity in rCutSmart Buffer
• Time-Saver™ qualified for digestion in 5-15 minutes
• Reduced star activity
• NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly.
• Can be used with NEBridge^® Ligase Master Mix for Golden Gate Assembly
• Restriction Enzyme Cut Site: GGTCTC(1/5)

Product Source

An E. coli strain that carries the cloned and modified BsaI gene from Bacillus stearothermophilus 6-55 (Z. Chen)