ZymoScript One-Step RT-qPCR Kit contains all the necessary components for fast reverse transcription and robust qPCR amplification in a single kit. Room-temperature reaction setup is quick and easy. The ZymoScript One-Step RT-qPCR Kit eliminates the need to transfer tubes between reverse transcription and qPCR, which minimizes sample handling and reduces the risk of contamination.
The included Reaction Buffer (4X) contains dNTPs, MgCl2, and other additives to ensure complete cDNA synthesis and specific qPCR amplification in just 1.5 hours. Non-interfering blue tracking dye is included to simplify reaction setup and prevent pipetting errors. The 20X Enzyme Mix containing temperature-activated reverse transcriptase, hot-start DNA polymerase, and RNase inhibitors ensures maximal specificity. The ZymoScript One-Step RT-qPCR kit is compatible with both SYBR-Green and TaqMan probe-based assays for ultimate flexibility. A 20X Fluor Dye for SYBR-Green based detection methods is included for added convenience. Detection of multiple targets in a single reaction (multiplex RT-qPCR) is feasible using TaqMan probes (not provided).
The ZymoScript One-Step RT-qPCR Kit has been rigorously tested to demonstrate superior sensitivity, specificity, and reproducibility. The kit can reliably quantify as little as 0.1 pg RNA (including difficult GC-rich targets) and outperforms other commercial kits. This kit was also used to develop a molecular diagnostic test for COVID-19 ranked third in sensitivity by the American FDA. The unique buffer system and temperature-activated enzymes facilitate the melting of secondary structures and ensure ultimate specificity for the intended product. ZymoScript One-Step RT-qPCR Kit is the product of choice for simple and sensitive RT-qPCR. It is the ideal product to use as a building block for RT-qPCR multiplex assays or high-throughput diagnostic applications. For quick and efficient cDNA synthesis and 2-step RT-qPCR options, see ZymoScript RT PreMix Kit (R3012) and ZymoTaq qPCR Premix (E2054).
Fast: Complete cDNA synthesis and robust amplification in as little as 1.5 hours
Flexible: Compatible with both SYBR-Green and TaqMan Probe-based assays
Efficient: Superior performance compared to competitor RT-qPCR kits
DESCRIPTION
ZymoScript One-Step RT-qPCR Kit contains all the necessary components for fast reverse transcription and robust qPCR amplification in a single kit. Room-temperature reaction setup is quick and easy. The ZymoScript One-Step RT-qPCR Kit eliminates the need to transfer tubes between reverse transcription and qPCR, which minimizes sample handling and reduces the risk of contamination.
The included Reaction Buffer (4X) contains dNTPs, MgCl2, and other additives to ensure complete cDNA synthesis and specific qPCR amplification in just 1.5 hours. Non-interfering blue tracking dye is included to simplify reaction setup and prevent pipetting errors. The 20X Enzyme Mix containing temperature-activated reverse transcriptase, hot-start DNA polymerase, and RNase inhibitors ensures maximal specificity. The ZymoScript One-Step RT-qPCR kit is compatible with both SYBR-Green and TaqMan probe-based assays for ultimate flexibility. A 20X Fluor Dye for SYBR-Green based detection methods is included for added convenience. Detection of multiple targets in a single reaction (multiplex RT-qPCR) is feasible using TaqMan probes (not provided).
The ZymoScript One-Step RT-qPCR Kit has been rigorously tested to demonstrate superior sensitivity, specificity, and reproducibility. The kit can reliably quantify as little as 0.1 pg RNA (including difficult GC-rich targets) and outperforms other commercial kits. This kit was also used to develop a molecular diagnostic test for COVID-19 ranked third in sensitivity by the American FDA. The unique buffer system and temperature-activated enzymes facilitate the melting of secondary structures and ensure ultimate specificity for the intended product. ZymoScript One-Step RT-qPCR Kit is the product of choice for simple and sensitive RT-qPCR. It is the ideal product to use as a building block for RT-qPCR multiplex assays or high-throughput diagnostic applications. For quick and efficient cDNA synthesis and 2-step RT-qPCR options, see ZymoScript RT PreMix Kit (R3012) and ZymoTaq qPCR Premix (E2054).
Zymo Research is ready to support your RNA studies and help simplify your workflow. Please contact tech@zymoresearch.com for more information.
TECHNICAL SPECIFICATIONS
Amplicon Length
This product performs best with amplicons between 50 bp and 1.5 kb
Equipment
thermal cycler/qPCR instrument
Ideal Uses
high-throughput applications, multiplex diagnostic assays based on RNA targets, routine quantification of specific targets in multiple samples
Processing Time
as little as 1.5 hours
Reagents
Complete and ready-to-use Reaction Buffer (4X) and Enzyme Mix (20X). Fluor Dye (20X) included for SYBR-Green based detection. MgCl2 included to supplement the magnesium concentration if needed.
This product can be used as a building block for molecular tests (e.g., for SARS-CoV-2 detection) and for research applications.
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Price
1.470,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS
Fast: Complete cDNA synthesis and robust amplification in as little as 1.5 hours
Flexible: Compatible with both SYBR-Green and TaqMan Probe-based assays
Efficient: Superior performance compared to competitor RT-qPCR kits
DESCRIPTION
ZymoScript One-Step RT-qPCR Kit contains all the necessary components for fast reverse transcription and robust qPCR amplification in a single kit. Room-temperature reaction setup is quick and easy. The ZymoScript One-Step RT-qPCR Kit eliminates the need to transfer tubes between reverse transcription and qPCR, which minimizes sample handling and reduces the risk of contamination.
The included Reaction Buffer (4X) contains dNTPs, MgCl2, and other additives to ensure complete cDNA synthesis and specific qPCR amplification in just 1.5 hours. Non-interfering blue tracking dye is included to simplify reaction setup and prevent pipetting errors. The 20X Enzyme Mix containing temperature-activated reverse transcriptase, hot-start DNA polymerase, and RNase inhibitors ensures maximal specificity. The ZymoScript One-Step RT-qPCR kit is compatible with both SYBR-Green and TaqMan probe-based assays for ultimate flexibility. A 20X Fluor Dye for SYBR-Green based detection methods is included for added convenience. Detection of multiple targets in a single reaction (multiplex RT-qPCR) is feasible using TaqMan probes (not provided).
The ZymoScript One-Step RT-qPCR Kit has been rigorously tested to demonstrate superior sensitivity, specificity, and reproducibility. The kit can reliably quantify as little as 0.1 pg RNA (including difficult GC-rich targets) and outperforms other commercial kits. This kit was also used to develop a molecular diagnostic test for COVID-19 ranked third in sensitivity by the American FDA. The unique buffer system and temperature-activated enzymes facilitate the melting of secondary structures and ensure ultimate specificity for the intended product. ZymoScript One-Step RT-qPCR Kit is the product of choice for simple and sensitive RT-qPCR. It is the ideal product to use as a building block for RT-qPCR multiplex assays or high-throughput diagnostic applications. For quick and efficient cDNA synthesis and 2-step RT-qPCR options, see ZymoScript RT PreMix Kit (R3012) and ZymoTaq qPCR Premix (E2054).
Zymo Research is ready to support your RNA studies and help simplify your workflow. Please contact tech@zymoresearch.com for more information.
TECHNICAL SPECIFICATIONS
Amplicon Length
This product performs best with amplicons between 50 bp and 1.5 kb
Equipment
thermal cycler/qPCR instrument
Ideal Uses
high-throughput applications, multiplex diagnostic assays based on RNA targets, routine quantification of specific targets in multiple samples
Processing Time
as little as 1.5 hours
Reagents
Complete and ready-to-use Reaction Buffer (4X) and Enzyme Mix (20X). Fluor Dye (20X) included for SYBR-Green based detection. MgCl2 included to supplement the magnesium concentration if needed.
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