R3012, ZymoScript RT PreMix Kit

ZymoScript RT PreMix Kit is an optimized reverse transcription kit containing all the necessary components for quick and efficient cDNA synthesis. Reaction setup can be completed at room temperature in less than one minute: simply add RNA to the ready-to-use RT PreMix. The RT PreMix includes a blue dye for easy tracking of pipetting, making reaction setup easier than ever. This dye ensures accurate reaction preparation and does not interfere with downstream analyses, including fluorescent detection methods.

The reverse transcription mix includes random hexamer and oligo dT primers, dNTPs, RNase inhibitors, reverse transcriptase, and an optimized reaction buffer for efficient, unbiased cDNA synthesis from as little as 0.1 pg to up to 5 µg of input RNA. Robust reverse transcription is achieved even with challenging templates that are GC-rich or contain secondary structures. ZymoScript RT PreMix is the kit of choice for studying the expression of a high number of targets from a single sample using 2-step RT-qPCR. For fast reverse transcription and robust qPCR amplification in a single tube, see ZymoScript One-Step RT-qPCR Kit (R3014).