The Zymo-Seq™ miRNA Library Kit is an innovative small RNA sequencing library prep kit that uses a single-adapter and circularization strategy to reduce ligation bias and provide accurate small RNA profiling from cell-free RNA (cfRNA) and total RNA. This simple, streamlined workflow minimizes hands-on time and uses gel-free removal of adapter dimers to generate stranded small RNA libraries from plasma, biofluids, cells, tissues, and more.
To prepare Zymo-Seq™ miRNA libraries, RNA is ligated to the single miRNA adapter and the ligated product is circularized. Excess adapter is blocked and removed with specialized magnetic beads that allow a completely gel-free workflow for input as low as 1 ng. The circularized miRNA-adapter product is then reverse transcribed. The resulting cDNA is amplified using Indexing PCR primers which add on Illumina-compatible adapter and unique dual index (UDI) sequences for cost-effective multiplexing. Everything needed to make sequencing-ready libraries, including indexing primers and SPRI beads, are included in the kit. It’s RNA-Seq made simple.
Accurate miRNA profiling: A unique single adapter and circularization strategy reduces ligation bias and increases miRNA detection.
Direct detection: Generates small RNA libraries without the need for small RNA enrichment.
Gel-Free: Removes adapter dimers from any input without lengthy gel excision.
DESCRIPTION
The Zymo-Seq™ miRNA Library Kit is an innovative small RNA sequencing library prep kit that uses a single-adapter and circularization strategy to reduce ligation bias and provide accurate small RNA profiling from cell-free RNA (cfRNA) and total RNA. This simple, streamlined workflow minimizes hands-on time and uses gel-free removal of adapter dimers to generate stranded small RNA libraries from plasma, biofluids, cells, tissues, and more.
To prepare Zymo-Seq™ miRNA libraries, RNA is ligated to the single miRNA adapter and the ligated product is circularized. Excess adapter is blocked and removed with specialized magnetic beads that allow a completely gel-free workflow for input as low as 1 ng. The circularized miRNA-adapter product is then reverse transcribed. The resulting cDNA is amplified using Indexing PCR primers which add on Illumina-compatible adapter and unique dual index (UDI) sequences for cost-effective multiplexing. Everything needed to make sequencing-ready libraries, including indexing primers and SPRI beads, are included in the kit. It’s RNA-Seq made simple.
TECHNICAL SPECIFICATIONS
Additional Components Required (Not Provided)
Molecular biology grade pure ethanol (> 95%)
Maximum Input
200 ng of total RNA, or RNA extracted from 200 µL of plasma or serum.
Minimum Input
1 ng of total RNA, or RNA extracted from 50 µL of plasma or serum.
Processing Time
2 hours hands-on, 8 hours total
Recommended Equipment
A 96-well Aluminum Cooler Block for PCR tubes or a 96-well PCR tube rack may be placed on ice for ease of reagent assembly and maintaining samples at 4°C. To process > 12 samples at once, a multichannel pipette with low-retention tips is recommended for mixing and the timely addition of pre-aliquoted reagents.
Recommended Input
100 ng of total RNA, or RNA extracted from 200 µL of plasma or serum.
Required Equipment
Thermal cycler, magnetic stand for 0.2 mL PCR tubes or 96-well plate, mini centrifuge, and micro centrifuge.
Sample Type
High integrity total RNA (RIN > 7) containing miRNA, or cell-free RNA extracted from plasma/serum samples not preserved with heparin. Small RNA enrichment is not necessary.
Sequencing Platform Compatibility
Libraries are compatible with all Illumina sequencing platforms.
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Price
43.275,00 RON (preturile sunt fara TVA)
Description
HIGHLIGHTS
Accurate miRNA profiling: A unique single adapter and circularization strategy reduces ligation bias and increases miRNA detection.
Direct detection: Generates small RNA libraries without the need for small RNA enrichment.
Gel-Free: Removes adapter dimers from any input without lengthy gel excision.
DESCRIPTION
The Zymo-Seq™ miRNA Library Kit is an innovative small RNA sequencing library prep kit that uses a single-adapter and circularization strategy to reduce ligation bias and provide accurate small RNA profiling from cell-free RNA (cfRNA) and total RNA. This simple, streamlined workflow minimizes hands-on time and uses gel-free removal of adapter dimers to generate stranded small RNA libraries from plasma, biofluids, cells, tissues, and more.
To prepare Zymo-Seq™ miRNA libraries, RNA is ligated to the single miRNA adapter and the ligated product is circularized. Excess adapter is blocked and removed with specialized magnetic beads that allow a completely gel-free workflow for input as low as 1 ng. The circularized miRNA-adapter product is then reverse transcribed. The resulting cDNA is amplified using Indexing PCR primers which add on Illumina-compatible adapter and unique dual index (UDI) sequences for cost-effective multiplexing. Everything needed to make sequencing-ready libraries, including indexing primers and SPRI beads, are included in the kit. It’s RNA-Seq made simple.
TECHNICAL SPECIFICATIONS
Additional Components Required (Not Provided)
Molecular biology grade pure ethanol (> 95%)
Maximum Input
200 ng of total RNA, or RNA extracted from 200 µL of plasma or serum.
Minimum Input
1 ng of total RNA, or RNA extracted from 50 µL of plasma or serum.
Processing Time
2 hours hands-on, 8 hours total
Recommended Equipment
A 96-well Aluminum Cooler Block for PCR tubes or a 96-well PCR tube rack may be placed on ice for ease of reagent assembly and maintaining samples at 4°C. To process > 12 samples at once, a multichannel pipette with low-retention tips is recommended for mixing and the timely addition of pre-aliquoted reagents.
Recommended Input
100 ng of total RNA, or RNA extracted from 200 µL of plasma or serum.
Required Equipment
Thermal cycler, magnetic stand for 0.2 mL PCR tubes or 96-well plate, mini centrifuge, and micro centrifuge.
Sample Type
High integrity total RNA (RIN > 7) containing miRNA, or cell-free RNA extracted from plasma/serum samples not preserved with heparin. Small RNA enrichment is not necessary.
Sequencing Platform Compatibility
Libraries are compatible with all Illumina sequencing platforms.
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