Limba
Romanian
Romanian
English|
A restriction endonuclease that recognizes the sequence TTA_^TAA.
- 100% activity in rCutSmart™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
- Restriction Enzyme Cut Site: TTA/TAA
Product Source
An E. coli strain that carries the modified cloned PsiI gene from Pseudomonas species SE-G49. PsiI-v2 is an engineered and improved version of PsiI.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions
1X rCutSmart™ Buffer
Incubate at 37°C
1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 25%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 10%
rCutSmart™ Buffer: 100%
Diluent Compatibility
- Diluent B
Storage Buffer
10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutes
Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive
Mai multe informatii
| Price | 704,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
A restriction endonuclease that recognizes the sequence TTA_^TAA.
Product Source An E. coli strain that carries the modified cloned PsiI gene from Pseudomonas species SE-G49. PsiI-v2 is an engineered and improved version of PsiI. Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Reaction Conditions 1X rCutSmart™ Buffer 1X rCutSmart™ Buffer Activity in NEBuffers NEBuffer™ r1.1: 25% Diluent Compatibility
Storage Buffer 10 mM Tris-HCl Heat Inactivation 65°C for 20 minutes Methylation Sensitivity dam methylation: Not Sensitive
|