R0661S, MspJ I, recombinant - 200 units

MspJI, an EpiMark^®, validated product is a modification-dependent endonuclease that recognizes ^mCNNR sites and generates a double-stranded DNA break on the 3´ side of the modified cytosine at N₉/N₁₃. The recognized cytosine modifications include C5-methylation (5-mC) and C5-hydroxymethylation (5-hmC).

This enzyme is provided with an Enzyme Activator Solution which may be used for efficient digestion by MspJI.

The most common epigenetic modifications found in eukaryotic organisms are methylation marks at CpG or CHG sites. A subset of these modified sites are recognized and cleaved by MspJI.

At fully methylated CpG sites:
5´ . . . Y N ^mC G N R . . . 3´
3´ . . . R N G ^mC N Y . . . 5´

or CHG sites:
5´ . . . Y ^mC H G R . . . 3´
3´ . . . R G D ^mC Y . . . 5´

R = A or G
Y = C or T
H = A or C or T (not G)
D = A or G or T (not C) 

MspJI recognizes each hemi-methylated site individually and cleaves bidirectionally to generate 32-base or 31-base fragments, respectively. These fragments contain the central methylated site and have 4-base 5´ overhangs at each end. MspJI does not cleave unmodified DNA.

Product Source

An E. coli strain that carries the synthetic MspJI gene from Mycobacterium species JLS.