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R0539S, Bbs I - 300 units
523,60 RON
A restriction endonuclease that recognizes the sequence GAAGACNN^NNNN_.
SKU
NEB_R0539S
Time-Saver™ qualified for digestion in 5-15 minutes
Used in Golden Gate Assembly
sgRNA Template Construction for CRISPR/Cas Genome Editing
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
High Fidelity (HF®) version available (NEB #R3539) supplied with rCutSmart™ Buffer
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Restriction Enzyme Cut Site: GAAGAC(2/6)
BbsI has a High Fidelity version BbsI-HF® (NEB #R3539).
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An E. coli strain that carries the cloned BbsI gene from Bacillus brevis.
| Price | 440,00 RON (preturile sunt fara TVA) |
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| Description |
Time-Saver™ qualified for digestion in 5-15 minutes BbsI has a High Fidelity version BbsI-HF® (NEB #R3539). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
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