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P8116S, NEBExpress E. coli Lysis Reagent - 100 ml
785,40 RON
NEBExpress E. coli Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of E. coli cells without denaturing soluble proteins.
SKU
NEB_P8116S
NEBExpress E. coli Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of E. coli cells without denaturing soluble proteins.
- Ready-to-use liquid that is stable at room temperature
- Gentle yet highly active formulation of detergents in Tris buffer
- Scalable lysis reactions from small to large bacterial cell pellets and compatible with high throughput workflows
- Suitable for extracting proteins, especially thermosensitive proteins vulnerable to mechanical lysis procedures
- Compatible with analyses such as SDS-PAGE, Western blots, activity assay, immunoprecipitation, and downstream purification
- Suitable for disrupting most Gram-negative bacterial cells
- Lysis efficiency increases 2-fold when used in combination with NEBExpress T4 Lysozyme (NEB# P8115)
- Fast and simple protocol: harvested cells are resuspended and briefly incubated in the Lysis Reagent to extract soluble proteins. Insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use and compatible with affinity resins.
- Small (100 ml) and large kits (5 x 100 ml) are suitable for lysing 20 to 100 grams of cells, respectively.
Mai multe informatii
| Price | 660,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
NEBExpress E. coli Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of E. coli cells without denaturing soluble proteins.
|