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P8115L, NEBExpress T4 Lysozyme - 5 x 200 µg
3.113,04 RON
NEBExpress® T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the β-1,4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria). It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites.
SKU
NEB_P8115L
NEBExpress® T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the β-1,4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria). It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites.
Source: An E. coli strain that carries the bacteriophage T4 gene e.
Properties & Usage
Storage Buffer
50 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
0.03% Poloxamer 188
50% Glycerol
pH 7.5 @ 25°C
Advantages and Features
- NEBExpress T4 Lysozyme is 200-fold more active than chicken egg white lysozyme.
- Lysis reactions are scalable and compatible with high throughput workflows.
- Lysis efficiency increases 2-fold when used in combination with NEBExpress E. coli Lysis Reagent (NEB #P8116S).
- Fast and non-mechanical bacterial lysis; the lysate is ready to use and compatible with affinity resins.
- Suitable for lysing thermosensitive proteins vulnerable to mechanical lysis procedures
- Compatible with downstream applications such as SDS-PAGE, Western blot, activity assays, immunoprecipitation, and downstream purification
- Small (200 µg) and large (5 x 200 µg) formats are suitable for lysing 7 or 35 grams of cells, respectively.
- Recombinant and animal free.
Mai multe informatii
| Price | 2.616,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
NEBExpress® T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the β-1,4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria). It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites. Properties & Usage Storage Buffer 50 mM Tris-HCl Advantages and Features
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