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N0378S, pMAL-c6T Vector - 10 µg
1.099,56 RON
The pMAL-c6T Vector provides a method for producing a protein expressed from a cloned gene or open reading frame. The cloned gene is inserted downstream from the malE gene of E. coli, which encodes maltose-binding protein (MBP), resulting in the expression of an MBP fusion protein in the cytoplasm.
SKU
NEB_N0378S
The pMAL-c6T Vector provides a method for producing a protein expressed from a cloned gene or open reading frame. The cloned gene is inserted downstream from the malE gene of E. coli, which encodes maltose-binding protein (MBP), resulting in the expression of an MBP fusion protein in the cytoplasm. The MBP has been engineered for tighter binding to amylose resin. The method uses the strong “tac” promoter and the malE translation initiation signals to give high-level expression of the cloned sequences, and a one-step purification of the fusion protein using MBP’s affinity for maltose. The vector expresses an N-terminal hexahistidine tagged malE gene (lacking its secretory signal sequence) followed by a multiple cloning site containing a TEV protease recognition sequence and stop codons in all three frames. This allows MBP to be cleaved from the protein of interest by TEV Protease after purification. The vector also carries the lacIq gene, which encodes for the Lac repressor. This keeps expression from Ptac low in the absence of IPTG induction.
Source: NEB-10 beta competent E. coli (pMAL-c6T)
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| Price | 924,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
The pMAL-c6T Vector provides a method for producing a protein expressed from a cloned gene or open reading frame. The cloned gene is inserted downstream from the malE gene of E. coli, which encodes maltose-binding protein (MBP), resulting in the expression of an MBP fusion protein in the cytoplasm. The MBP has been engineered for tighter binding to amylose resin. The method uses the strong “tac” promoter and the malE translation initiation signals to give high-level expression of the cloned sequences, and a one-step purification of the fusion protein using MBP’s affinity for maltose. The vector expresses an N-terminal hexahistidine tagged malE gene (lacking its secretory signal sequence) followed by a multiple cloning site containing a TEV protease recognition sequence and stop codons in all three frames. This allows MBP to be cleaved from the protein of interest by TEV Protease after purification. The vector also carries the lacIq gene, which encodes for the Lac repressor. This keeps expression from Ptac low in the absence of IPTG induction. Source: NEB-10 beta competent E. coli (pMAL-c6T) |