Limba
Romanian
Romanian
English|
Authenticase is a proprietary mixture of structure-specific nucleases capable of recognizing and cleaving outside mismatch and indel (insertion and/or deletion) regions, ranging from 1-10 basepairs (bp) on double-stranded DNA. The formulation has limited non-specific activity on homoduplex regions of DNA. Authenticase can be used as an error-correction reagent in oligo-based PCR gene assembly by enzymatically removing “mistakes” prior to the final renaturation and amplification step (i.e. removes mismatch/indel errors caused by oligonucleotide synthesis). Alternatively, Authenticase can replace T7 Endonuclease I in the mismatch detection assay used to assess the efficiency of genome editing.
Reaction Conditions
1X Authenticase Reaction Buffer
Incubate at 42°C
1X Authenticase Reaction Buffer
10 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 8 @ 25°C)
Storage Buffer
10 mM Tris-HCl
500 mM NaCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
No
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| Price | 3.168,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Authenticase is a proprietary mixture of structure-specific nucleases capable of recognizing and cleaving outside mismatch and indel (insertion and/or deletion) regions, ranging from 1-10 basepairs (bp) on double-stranded DNA. The formulation has limited non-specific activity on homoduplex regions of DNA. Authenticase can be used as an error-correction reagent in oligo-based PCR gene assembly by enzymatically removing “mistakes” prior to the final renaturation and amplification step (i.e. removes mismatch/indel errors caused by oligonucleotide synthesis). Alternatively, Authenticase can replace T7 Endonuclease I in the mismatch detection assay used to assess the efficiency of genome editing. Reaction Conditions 1X Authenticase Reaction Buffer 1X Authenticase Reaction Buffer Storage Buffer 10 mM Tris-HCl Heat Inactivation No |