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Enzymatic methods for DNA fragmentation in NGS workflows enable streamlined protocols, improved performance and scalability. However, specialized fragmentation reagents are required for samples for methylation analysis, to ensure that methylation marks are not removed, and for FFPE DNA. NEBNext UltraShear is a novel enzyme mix designed for fragmentation of these sample types that has a fast workflow and improves library preparation and sequencing metrics for DNA methylation studies and FFPE DNA.
NEBNext UltraShear is compatible with NEBNext Enzymatic Methyl-seq (EM-seq) (NEB #E7120).
NEBNext UltraShear in FFPE DNA sequencing workflows:
- Improved usable reads
- Lower artificial mutation frequency
NEBNext UltraShear for methylated DNA sequencing e.g., EM-seq
- Higher library yields
- Improved sequencing metrics
- Improved CpG Coverage
Note that for high quality genomic DNA library prep with enzymatic fragmentation, we recommend NEBNext Ultra II FS DNA Library Prep for Illumina (NEB #E7805, #E6177).
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| Price | 2.760,00 RON (preturile sunt fara TVA) |
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| Description |
Enzymatic methods for DNA fragmentation in NGS workflows enable streamlined protocols, improved performance and scalability. However, specialized fragmentation reagents are required for samples for methylation analysis, to ensure that methylation marks are not removed, and for FFPE DNA. NEBNext UltraShear is a novel enzyme mix designed for fragmentation of these sample types that has a fast workflow and improves library preparation and sequencing metrics for DNA methylation studies and FFPE DNA.
NEBNext UltraShear for methylated DNA sequencing e.g., EM-seq
Note that for high quality genomic DNA library prep with enzymatic fragmentation, we recommend NEBNext Ultra II FS DNA Library Prep for Illumina (NEB #E7805, #E6177).
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