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T1130L, Monarch Spin PCR & DNA Cleanup Kit (5ug) - 250 preps
3.049,20 RON
Monarch® Spin PCR & DNA Cleanup Kit (5 μg)
- New version of PCR and DNA cleanup kit, featuring upgraded spin columns precision-engineered for high performance
- Elute in as little as 5 μl for highly concentrated DNA, with yields up to 5 μg
- Prevent buffer retention and salt carry over with unique, optimized column design
- No need to monitor pH
- Protocol modification for oligonucleotide cleanup is provided, allowing purification of ssDNA, oligonucleotide and other small DNA fragments
- Reduce hands-on time with faster protocols and less spin time
- Significantly less plastic by design in columns and kit compared to leading suppliers
SKU
NEB_T1130L
The Monarch Spin PCR & DNA Cleanup Kit (5 μg) offers an efficient method for purification and concentration up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 μl, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. The kit includes spin columns, collection tubes, buffers, and other reagents in compact and streamlined packaging for silica membrane-based PCR purification and DNA cleanup. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps. The kit features Monarch spin columns that are manufactured using state of the art automation machinery for high quality and consistency.
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Properties |
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Purification format |
Spin-column |
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DNA sample |
DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations) |
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ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol |
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Typical recovery |
70-90% |
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DNA Purity |
A260/280 > 1.8 and A260/230 > 1.8 |
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Binding Capacity |
Up to 5 μg |
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Elution Volume |
5-20 μl |
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DNA Size Range |
Standard protocol: 50 bp – 25 kb |
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Protocol Time |
5 minutes of spin and incubation time |
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Compatible Downstream Applications |
Ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing |
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| Price | 2.520,00 RON (preturile sunt fara TVA) | |||||||||||||||||||||
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| Description |
The Monarch Spin PCR & DNA Cleanup Kit (5 μg) offers an efficient method for purification and concentration up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 μl, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. The kit includes spin columns, collection tubes, buffers, and other reagents in compact and streamlined packaging for silica membrane-based PCR purification and DNA cleanup. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps. The kit features Monarch spin columns that are manufactured using state of the art automation machinery for high quality and consistency.
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