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M0665S, Tth Argonaute (TtAgo) - 50 pmol
621,18 RON
Thermus thermophilus argonaute (TtAgo) is a programmable DNA-endonuclease which requires a short 5’-phosphorylated single-stranded DNA guide to target its activity to a specific corresponding sequence on a substrate. TtAgo introduces one break in the phosphodiester backbone of the complementary substrate sequence.
SKU
NEB_M0665S
Thermus thermophilus argonaute (TtAgo) is a programmable DNA-endonuclease which requires a short 5’-phosphorylated single-stranded DNA guide to target its activity to a specific corresponding sequence on a substrate. TtAgo introduces one break in the phosphodiester backbone of the complementary substrate sequence.
Short 16–18 nucleotide 5′-phosphorylated ssDNA guides are cost effective and can be phosphorylated with NEB T4 Polynucleotide Kinase
Guide/target sequence selection is not limited by the requirement of an adjacent sequence motif
Highly active on ssDNA and most dsDNA substrates (generates a nick in dsDNA substrates), with mild activity on ssRNA substrates
Recommended for in vitro applications
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| Price | 522,00 RON (preturile sunt fara TVA) |
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| Description |
Thermus thermophilus argonaute (TtAgo) is a programmable DNA-endonuclease which requires a short 5’-phosphorylated single-stranded DNA guide to target its activity to a specific corresponding sequence on a substrate. TtAgo introduces one break in the phosphodiester backbone of the complementary substrate sequence. Short 16–18 nucleotide 5′-phosphorylated ssDNA guides are cost effective and can be phosphorylated with NEB T4 Polynucleotide Kinase
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