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T5 Exonuclease degrades DNA in the 5´ to 3´ direction. T5 Exonuclease is able to initiate nucleotide removal from the 5′ termini or at gaps and nicks of linear or circular dsDNA. However, the enzyme does not degrade supercoiled dsDNA. T5 Exonuclease also has ssDNA endonuclease activity.
Product Source
The T5 phage D15 gene is expressed as His-tag fusion in E. coli.
Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease
Initiates at the 5' termini of linear or nicked double-stranded DNA
Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction
T5 Exonuclease is ideal for:
Removal of incomplete ligation products from ligated circular double-stranded DNA
Degradation of denatured DNA from alkaline-based plasmid purification methods for improved DNA cloning
Degradation of contaminating linear and nicked DNA in plasmid samples
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| Price | 1.683,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
T5 Exonuclease degrades DNA in the 5´ to 3´ direction. T5 Exonuclease is able to initiate nucleotide removal from the 5′ termini or at gaps and nicks of linear or circular dsDNA. However, the enzyme does not degrade supercoiled dsDNA. T5 Exonuclease also has ssDNA endonuclease activity. Product Source
Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease T5 Exonuclease is ideal for: Removal of incomplete ligation products from ligated circular double-stranded DNA |