Limba
Romanian
Romanian
English|
mRNA Decapping Enzyme catalyzes the removal of 7-methylguanosine cap (m7G) from 5´ end of mRNA, producing 5′ monophosphate and releasing m7GDP. mRNA Decapping Enzyme is capable of decapping mRNAs of various lengths and removes both Cap0 and Cap1 structures with similar efficiency. mRNA Decapping Enzyme also converts 5′ triphosphate ends to 5′ monophosphate, albeit with reduced efficiency. 5′ monophosphorylated RNA can be exploited in a variety of downstream applications, including 5′ RNA Ligase-mediated RACE, RNA-seq, and 5′-> 3′ exonuclease digestion.
Product Source
mRNA Decapping Enzyme from S.pombe is expressed as a His-tagged fusion in E. coli.
Unit Definition
One unit is defined as the amount of mRNA Decapping Enzyme required to convert 50% of a 500 nM m7G-capped substrate to a 5´-monophosphorylated form in a total reaction volume of 20 µl in 1 hour at 37˚C.
Reaction Conditions
1X mRNA Decapping Enzyme Reaction Buffer
Incubate at 37°C
1X mRNA Decapping Enzyme Reaction Buffer
50 mM Tris-HCl
50 mM Ammonium Chloride
5 mM MgCl2
1 mM DTT
0.1% Poloxamer 188
(pH 7.5 @ 25°C)
Storage Buffer
300 mM NaCl
10 mM Tris-HCl
0.1 mM DTT
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
No
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| Price | 672,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
mRNA Decapping Enzyme catalyzes the removal of 7-methylguanosine cap (m7G) from 5´ end of mRNA, producing 5′ monophosphate and releasing m7GDP. mRNA Decapping Enzyme is capable of decapping mRNAs of various lengths and removes both Cap0 and Cap1 structures with similar efficiency. mRNA Decapping Enzyme also converts 5′ triphosphate ends to 5′ monophosphate, albeit with reduced efficiency. 5′ monophosphorylated RNA can be exploited in a variety of downstream applications, including 5′ RNA Ligase-mediated RACE, RNA-seq, and 5′-> 3′ exonuclease digestion. Product Source mRNA Decapping Enzyme from S.pombe is expressed as a His-tagged fusion in E. coli. Unit Definition One unit is defined as the amount of mRNA Decapping Enzyme required to convert 50% of a 500 nM m7G-capped substrate to a 5´-monophosphorylated form in a total reaction volume of 20 µl in 1 hour at 37˚C. Reaction Conditions 1X mRNA Decapping Enzyme Reaction Buffer 1X mRNA Decapping Enzyme Reaction Buffer Storage Buffer 300 mM NaCl Heat Inactivation No
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