M0607S, NudC Pyrophosphatase - 250 pmol

606,90 RON

SKU

NEB_M0607S

NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD⁺- and NADH-capped RNA, generating a ligatible 5′ monophosphate on the RNA (NAD⁺ decapping or deNADding). Deletion of the nudC gene has been shown to increase the fraction of NAD⁺-capped RNA in E. coli.

NudC has also been shown to hydrolyze small molecules containing an ADP moiety such as NADH, NAD⁺, AppA, ADP-ribose, ADP-glucose and metabolic co-factors such as FAD, Coenzyme A and Acetyl CoA at varying efficiency. With its activity on various dinucleotides and metabolic co-factors, NudC may potentially be used to identify RNA carrying non-canonical caps by pyrophosphorolysis of the cap and analysis of the product nucleotide, or identifying the resulting 5' monophosphate end of the RNA using 5^’ ligation-based methods such as RNA-seq or 5’ RACE.

Unit Definition

1 µM of NudC hydrolyzes 200 µM or more NAD⁺ into NMN⁺ and AMP in 1X NEBuffer 3.1 and 5 mM DTT at 37°C for 30 min.

Reaction Conditions

1X NEBuffer™ r3.1
Supplement with 5 mM DTT
Incubate at 37°C

NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Storage Buffer

300 mM NaCl
10 mM Tris-HCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.9 @ 25°C

Heat Inactivation

65°C for 10 minutes

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Price	510,00 RON (preturile sunt fara TVA)
Description	NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD⁺- and NADH-capped RNA, generating a ligatible 5′ monophosphate on the RNA (NAD⁺ decapping or deNADding). Deletion of the nudC gene has been shown to increase the fraction of NAD⁺-capped RNA in E. coli. NudC has also been shown to hydrolyze small molecules containing an ADP moiety such as NADH, NAD⁺, AppA, ADP-ribose, ADP-glucose and metabolic co-factors such as FAD, Coenzyme A and Acetyl CoA at varying efficiency. With its activity on various dinucleotides and metabolic co-factors, NudC may potentially be used to identify RNA carrying non-canonical caps by pyrophosphorolysis of the cap and analysis of the product nucleotide, or identifying the resulting 5' monophosphate end of the RNA using 5^’ ligation-based methods such as RNA-seq or 5’ RACE. Unit Definition 1 µM of NudC hydrolyzes 200 µM or more NAD⁺ into NMN⁺ and AMP in 1X NEBuffer 3.1 and 5 mM DTT at 37°C for 30 min. Reaction Conditions 1X NEBuffer™ r3.1 Supplement with 5 mM DTT Incubate at 37°C NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl₂ 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Storage Buffer 300 mM NaCl 10 mM Tris-HCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.9 @ 25°C Heat Inactivation 65°C for 10 minutes

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M0607S, NudC Pyrophosphatase - 250 pmol

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