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Quick CIP is a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.
- Rapid and irreversible heat inactivation eliminates unwanted activity
- Improved storage stability versus native enzyme
- Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
- Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
- Less enzyme required (high specific activity), resulting in a lower cost per reaction
- No need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )
- Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis
- Recombinant for purity, consistency and value
Product Source
A Pichia pastoris clone that carries the calf intestinal alkaline phosphatase gene from calf intestinal mucosa.
- Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of p-Nitrophenyl Phosphate, PNPP (NEB #P0757) in a total reaction volume of 1 ml in 1 minute at 37°C.
Reaction Conditions
1X rCutSmart™ Buffer
Incubate at 37°C
rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Storage Buffer
25 mM Tris-HCl
1 mM MgCl2
0.1 mM ZnCl2
50% Glycerol
pH 7.5 @ 25°C
Heat Inactivation
80°C for 2 minutes
Unit Assay Conditions
1 M diethanolamine-HCl (pH 9.8), 0.5 mM MgCl2, 50 mM p-Nitrophenyl Phosphate. These conditions are only used for quantitating enzyme activity.
- Advantages and Features
Features
- Rapid and irreversible heat inactivation eliminates unwanted activity
- Improved storage stability versus native enzyme
- Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
- Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
- Less enzyme required (high specific activity), resulting in a lower cost per reaction
- No need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )
- Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis
- Recombinant for purity, consistency and value
Application Features
- Dephosphorylation 5′ and 3′ ends of DNA and RNA.
- Dephosphorylation of cloning vector DNA to prevent recircularization during ligation.
- Dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase.
- Removal of dNTPs in PCR reactions prior to sequencing or SNP analysis.
Mai multe informatii
| Price | 572,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Quick CIP is a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.
Product Source A Pichia pastoris clone that carries the calf intestinal alkaline phosphatase gene from calf intestinal mucosa.
One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of p-Nitrophenyl Phosphate, PNPP (NEB #P0757) in a total reaction volume of 1 ml in 1 minute at 37°C. Reaction Conditions 1X rCutSmart™ Buffer rCutSmart™ Buffer Storage Buffer 25 mM Tris-HCl Heat Inactivation 80°C for 2 minutes Unit Assay Conditions 1 M diethanolamine-HCl (pH 9.8), 0.5 mM MgCl2, 50 mM p-Nitrophenyl Phosphate. These conditions are only used for quantitating enzyme activity.
Features
Application Features
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