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E1603L, phi29-XT RCA Kit - 500 rxns
10.738,56 RON
The phi29-XT RCA Kit includes everything needed for sensitive and robust rolling circle amplification (RCA), an isothermal amplification method to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence. The kit features phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability and sensitivity, and generates higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase.
SKU
NEB_E1603L
The phi29-XT RCA Kit includes everything needed for sensitive and robust rolling circle amplification (RCA), an isothermal amplification method to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence. The kit features phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability and sensitivity, and generates higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are dNTPs and exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify circular DNA sequences. Input material can be purified circular DNA (single or double-stranded) or direct from liquid media culture, agar plate colonies, and glycerol stocks without the need for DNA extraction. RCA products can be used directly in downstream applications such as DNA sequencing, cell-free DNA enrichment, cell-free protein expression, and DNA biosensors.
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| Price | 9.024,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
The phi29-XT RCA Kit includes everything needed for sensitive and robust rolling circle amplification (RCA), an isothermal amplification method to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence. The kit features phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability and sensitivity, and generates higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are dNTPs and exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify circular DNA sequences. Input material can be purified circular DNA (single or double-stranded) or direct from liquid media culture, agar plate colonies, and glycerol stocks without the need for DNA extraction. RCA products can be used directly in downstream applications such as DNA sequencing, cell-free DNA enrichment, cell-free protein expression, and DNA biosensors. |