ELK9884-48T, Human DENV NS1 (Dengue virus non-structural protein 1) ELISA Kit, 48T

Alternative Names: Dengue virus non-structural protein 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.65 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, tissue homogenates

Assay length: 3.5h

Research Area: Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DENV NS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DENV NS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DENV NS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DENV NS1 in the samples is then determined by comparing the OD of the samples to the standard curve.