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ELK9878-96T, Rat PGF2α(Prostaglandin F2 Alpha) ELISA Kit, 96T
2.963,10 RON
Rat PGF2α(Prostaglandin F2 Alpha) ELISA Kit
SKU
ELK9878-96T
Alternative Names: 8-isoprostane; 8-iso-PGF2α
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 3.88 pg/mL
Standard: 800 pg/mL
Detection range: 12.5-800 pg/mL
Sample type: Serum, plasma, saliva and other biological fluids.
Assay length: 2h
Research Area: Metabolic pathway;Cardiovascular biology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: 8-isoprostane; 8-iso-PGF2α Species: Rat Assay Type: Competitive Inhibition Sensitivity: 3.88 pg/mL Standard: 800 pg/mL Detection range: 12.5-800 pg/mL Sample type: Serum, plasma, saliva and other biological fluids. Assay length: 2h Research Area: Metabolic pathway;Cardiovascular biology;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve. |
