ELK9869-96T, Human RANKL(Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit, 96T

Alternative Names: CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine; RANkL; Receptor Activator Of Nuclear Factor Kappa B Ligand

Species: Human

Assay Type: Sandwich

Sensitivity: 22.7 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Metabolic pathway;Apoptosis;Tumor immunity;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RANKL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RANKL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RANKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RANKL in the samples is then determined by comparing the OD of the samples to the standard curve.