ELK9867-48T, Rat FBXO32(F-Box Protein 32) ELISA Kit, 48T

Alternative Names: ATROGIN1; Fbx32; MAFbx; Muscle atrophy F-box protein

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.57 pg/mL

Standard: 100 pg/mL

Detection range: 1.57-100 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FBXO32. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FBXO32. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FBXO32, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FBXO32 in the samples is then determined by comparing the OD of the samples to the standard curve.