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ELK9826-96T, Human Adalimumab ELISA Kit, 96T
2.963,10 RON
Human Adalimumab ELISA Kit
SKU
ELK9826-96T
Alternative Names: Adalimumab
Species: Human
Assay Type: Sandwich
Sensitivity: 4.93 ng/mL
Standard: 1000 ng/mL
Detection range: 15.63-1000 ng/mL
Sample type: serum, plasma
Assay length: 3.5h
Research Area: Immunology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Adalimumab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Adalimumab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Adalimumab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Adalimumab in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Adalimumab Species: Human Assay Type: Sandwich Sensitivity: 4.93 ng/mL Standard: 1000 ng/mL Detection range: 15.63-1000 ng/mL Sample type: serum, plasma Assay length: 3.5h Research Area: Immunology Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Adalimumab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Adalimumab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Adalimumab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Adalimumab in the samples is then determined by comparing the OD of the samples to the standard curve. |
