PGE1(Prostaglandin E1) ELISA Kit
Alternative Names: Prostaglandin E1; PGE1
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 9.55 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine
Assay length: 2h
Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGE1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.080,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: Prostaglandin E1; PGE1 Species: General Assay Type: Competitive Inhibition Sensitivity: 9.55 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine Assay length: 2h Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGE1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve. |