ELK9815-48T, PGE1(Prostaglandin E1) ELISA Kit, 48T

ELK9815-48T, PGE1(Prostaglandin E1) ELISA Kit, 48T

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ELK9815-96T, PGE1(Prostaglandin E1) ELISA Kit, 96T

2.475,20 RON

PGE1(Prostaglandin E1) ELISA Kit

SKU
ELK9815-96T

Alternative Names: Prostaglandin E1; PGE1

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 9.55 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine

Assay length: 2h

Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGE1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Prostaglandin E1; PGE1

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 9.55 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine

Assay length: 2h

Research Area: Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGE1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.