ELK9793-96T, Rabbit Vaspin(Visceral Adipose Tissue Derived Serine Protease Inhibitor) ELISA Kit, 96T

Alternative Names: SERPINA12; OL-64; Visceral Adipose-Specific SERPIN; Serine Proteinase Inhibitor,Clade A Member 12; Visceral adipose-specific serpin

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 35.19 pg/mL

Standard: 6000 pg/mL

Detection range: 93.75-6000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Metabolic pathway;Endocrinology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit Vaspin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit Vaspin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit Vaspin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit Vaspin in the samples is then determined by comparing the OD of the samples to the standard curve.