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ELK9778-48T, Rabbit dsDNA(anti-double stranded DNA antibody IgM) ELISA Kit, 48T
2.142,00 RON
Rabbit dsDNA(anti-double stranded DNA antibody IgM) ELISA Kit
SKU
ELK9778-48T
Alternative Names: dsDNA Ab IgM
Species: Rabbit
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: serum, plasma, urine and other biological fluids
Assay length: 3.5h
Research Area: Disease
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit dsDNA IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit dsDNA IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit dsDNA IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit dsDNA IgM in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: dsDNA Ab IgM Species: Rabbit Assay Type: Sandwich Sensitivity: 0.58 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: serum, plasma, urine and other biological fluids Assay length: 3.5h Research Area: Disease Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit dsDNA IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit dsDNA IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit dsDNA IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit dsDNA IgM in the samples is then determined by comparing the OD of the samples to the standard curve. |
