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ELK9773-96T, Dog SPD(Surfactant Associated Protein D) ELISA Kit, 96T
2.963,10 RON
Dog SPD(Surfactant Associated Protein D) ELISA Kit
SKU
ELK9773-96T
Alternative Names: SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D
Species: Dog
Assay Type: Sandwich
Sensitivity: 12.7 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma, tissue homogenates, lung lavage fluid and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog SPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog SPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SPD in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D Species: Dog Assay Type: Sandwich Sensitivity: 12.7 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: serum, plasma, tissue homogenates, lung lavage fluid and other biological fluids Assay length: 3.5h Research Area: Infection immunity;Pulmonology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog SPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog SPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SPD in the samples is then determined by comparing the OD of the samples to the standard curve. |
