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ELK9771-48T, Dog FPR1(Formyl Peptide Receptor 1) ELISA Kit, 48T
2.142,00 RON
Dog FPR1(Formyl Peptide Receptor 1) ELISA Kit
SKU
ELK9771-48T
Alternative Names: FMLP; FPR; fMet-Leu-Phe receptor; N-formylpeptide chemoattractant receptor
Species: Dog
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog FPR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog FPR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog FPR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog FPR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: FMLP; FPR; fMet-Leu-Phe receptor; N-formylpeptide chemoattractant receptor Species: Dog Assay Type: Sandwich Sensitivity: 0.061 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Endocrinology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog FPR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog FPR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog FPR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog FPR1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
