ELK9767-96T, Horse IgE(Immunoglobulin E) ELISA Kit, 96T

ELK9767-96T, Horse IgE(Immunoglobulin E) ELISA Kit, 96T

ELK9768-96T, Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit, 96T

ELK9768-96T, Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit, 96T

ELK9768-48T, Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit, 48T

2.142,00 RON

Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit

SKU
ELK9768-48T

Alternative Names: Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Species: Dog

Assay Type: Competitive Inhibition

Sensitivity: 4.25 µg/mL

Standard: 1000 µg/mL

Detection range: 15.63-1000 µg/mL

Sample type: plasma and erythrocyte lysates

Assay length: 2h

Research Area: Diagnosis of diabetes mellitus

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Species: Dog

Assay Type: Competitive Inhibition

Sensitivity: 4.25 µg/mL

Standard: 1000 µg/mL

Detection range: 15.63-1000 µg/mL

Sample type: plasma and erythrocyte lysates

Assay length: 2h

Research Area: Diagnosis of diabetes mellitus

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.