ELK9758-96T, Human UNC5B(Netrin Receptor UNC5B) ELISA Kit, 96T

Alternative Names: Netrin receptor UNC5B; Protein unc-5 homolog 2; Protein unc-5 homolog B; p53-regulated receptor for death and life protein 1; p53RDL1; UNC5B; P53RDL1; UNC5H2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Neuroscience; Cardiovascular

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UNC5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UNC5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UNC5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UNC5B in the samples is then determined by comparing the OD of the samples to the standard curve.