ELK9748-48T, Pig MIP1b(Macrophage Inflammatory Protein 1 Beta) ELISA Kit, 48T

Alternative Names: MIP1 Beta; CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b; CCL4L1; C C motif chemokine ligand 4 like 1; CC chemokine ligand 4L1; C-C motif chemokine 4-like; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; Monocyte adherence-induced protein 5-alpha; Small-inducible cytokine A4-like; CCL4L; SCYA4L1; CCL4L2; SCYA4L2

Species: Pig

Assay Type: Sandwich

Sensitivity: 30 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay length: 3.5h

Research Area: Cytokine;Infection immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve.