ELK9741-48T, Cattle MYO(Myoglobin) ELISA Kit, 48T

ELK9741-48T, Cattle MYO(Myoglobin) ELISA Kit, 48T

ELK9742-48T, Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit, 48T

ELK9742-48T, Cattle NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit, 48T

ELK9741-96T, Cattle MYO(Myoglobin) ELISA Kit, 96T

3.736,60 RON

Cattle MYO(Myoglobin) ELISA Kit

SKU
ELK9741-96T

Alternative Names: PVALB; MB

Species: Cattle

Assay Type: Sandwich

Sensitivity: 0.31 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle MYO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle MYO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle MYO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle MYO in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: PVALB; MB

Species: Cattle

Assay Type: Sandwich

Sensitivity: 0.31 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle MYO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle MYO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle MYO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle MYO in the samples is then determined by comparing the OD of the samples to the standard curve.