Rat OT(Oxytocin) ELISA Kit
Alternative Names: Pitocin; Syntocinon
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 5.51 pg/mL
Standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Reproductive science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat OT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OT in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 2.080,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: Pitocin; Syntocinon Species: Rat Assay Type: Competitive Inhibition Sensitivity: 5.51 pg/mL Standard: 1000 pg/mL Detection range: 15.63-1000 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Reproductive science; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat OT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OT in the samples is then determined by comparing the OD of the samples to the standard curve. |